Metagenomics sequencing

Service Introduction

Metagenomics sequencing is the high-throughput sequencing of the genomes of microbial communities in a specific environmental sample. It is used to analyze the composition and function of microbial communities, to understand the diversity and abundance of microbial communities, to explore the relationship between microbes and the environment, and to discover and study new genes with specific functions.

Based on high-throughput sequencing, metagenomics research has the following advantages:

Many microorganisms in the natural world cannot be cultured in the laboratory, thus expanding the scope of microbial research without the need for isolation and cultivation.

The method studies the macroscopic features of microbial community diversity, functional genes, etc. in the environment, which more quickly, efficiently, and accurately reflects the true state of microorganisms.

Without the need for enrichment of genomic fragments in host bacteria, high-throughput sequencing can analyze and explore the species diversity and functional genes in the total microbial community, promoting the wide application of metagenomics in a variety of research fields.

Platform advantages

Short cycle: After receiving customer samples, the cycle from quality inspection to the end of bioinformatics analysis is generally controlled within 30-45 working days, and the completion cycle will be adjusted according to the number of samples in the project.

Experienced technical staff: Can provide metagenomics and multi-omics experimental plans according to the requirements of partners, and solve experimental problems.

Technical Procedures

Bioinformatics Analysis

生物信息學(xué)分析
宏基因組細(xì)菌多樣性測(cè)序分析內(nèi)容	宏基因組學(xué)分析內(nèi)容
總體數(shù)據(jù)統(tǒng)計(jì)分析	將 reads 與已測(cè)序微生物基因組（或參考序列）進(jìn)行比對(duì)，得到物種分類(lèi)信息以及每個(gè)物種上的 reads 個(gè)數(shù)統(tǒng)計(jì)
單個(gè)樣品中微生物種類(lèi)及豐度分析，序列聚類(lèi) OTU，群落結(jié)構(gòu)分析	對(duì) reads 組裝，得到 contigs 序列，根據(jù) contig 的拼接結(jié)果，進(jìn)行基因預(yù)測(cè)、基因功能注釋、COG 分類(lèi)、GO 分類(lèi)、KEGG-KO 分類(lèi)及KEGG-map 代謝圖
多個(gè)樣品間比較分析，包括相似度比對(duì)，OTU 比較、多樣品群落結(jié)構(gòu)分析，PCA（主成分）分析

Sample Requirements

實(shí)驗(yàn)類(lèi)型	樣品總量	樣品濃度	樣品純度OD260/280	DNA質(zhì)量	樣品保存	樣品運(yùn)輸
環(huán)境細(xì)菌多樣性	1ug DNA	>50 ng/μl	1.8-2.0,無(wú)蛋白質(zhì)、RNA 或肉眼可見(jiàn)雜質(zhì)污染	基因組完整、無(wú)降解，電泳結(jié)果基因組 DNA 主帶在預(yù)期位置，清晰，無(wú)彌散，cDNA 片段化樣品，片段大小符合預(yù)期位置	請(qǐng)選擇干粉、酒精、TE buffer 或超純水一種，并在樣品信息單中注明	請(qǐng)將樣品置于 1.5 ml 管中，做好標(biāo)記，并用封口膜好，并同《測(cè)序樣品訂單》一起放在塑料樣品袋封口，基因組 DNA 如果用乙醇沉淀，可以常溫運(yùn)輸。
宏基因組	3ug DNA	>30 ng/μl	1.8-2.0,無(wú)蛋白質(zhì)、RNA 或肉眼可見(jiàn)雜質(zhì)污染		請(qǐng)選擇干粉、酒精、TE buffer 或超純水一種，并在樣品信息單中注明