Small RNA sequencing
Service introduction
Small RNAs (sRNA) are important regulators of life activities, playing important roles in gene expression regulation, individual development, metabolism, and the occurrence of diseases. The Illumina sequencing platform can perform deep sequencing of all sRNA in the sample to achieve qualitative and quantitative research purposes. Each sample can obtain more than 10 million sRNA sequencing sequences. Through massive parallel sequencing, it can discover, identify, and quantify the small RNA map at the whole genome level of any species, the excavation of new mRNA molecules, the prediction of target genes, identification, and differential expression analysis between samples.
Technical procedures
Sample requirements
實(shí)驗(yàn)類(lèi)型 | RNA樣品總量 | 樣品濃度和純度 | 樣品質(zhì)量 | 樣品保存 | 樣品運(yùn)輸 |
Small RNA測(cè)序 | 一次性提供至少3 μg高質(zhì)量的總 RNA | 樣品濃度 >100 ng/μl,OD260/280 介于 1.8-2.0 之間,無(wú)肉眼可見(jiàn)污染 | 使用 Agilent Bioanalyzer 儀器檢測(cè),則獲得的總的 RNA 28S:18S >1,RIN>7.5 | 請(qǐng)選擇乙醇、DEPC 水保存樣品,并在樣品信息單中注明 | 樣品請(qǐng)置于 1.5 ml 管中,并使用封口膜封好,放置足夠的干冰運(yùn)輸 |
Experiment cycle and sequencing strategy
實(shí)驗(yàn)類(lèi)型 | 測(cè)序策略 | 數(shù)據(jù)量 | 實(shí)驗(yàn)周期 |
Small RNA測(cè)序 | HiseqSE50 | 10M | 樣品質(zhì)檢合格后,30-50 個(gè)工作日 |