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Long non-coding RNA sequencing service

Service introduction

Long non-coding RNA (lncRNA) is a class of RNA molecules that are longer than 200 nt and do not encode proteins. They regulate gene expression at the epigenetic, transcriptional, and post-transcriptional levels by binding to DNA, RNA, or proteins. lncRNA sequencing is used to study all lncRNA and mRNA transcripts that are expressed in a specific tissue or cell type of a species with an available reference genome at a particular time point.

Experimental procedures

Technical procedures

Sample requirements

實(shí)驗(yàn)類型	RNA總量	樣品濃度和純度	樣品質(zhì)量	樣品保存	樣品運(yùn)輸	樣品選擇
lncRNA測(cè)序	≥ 3.0μg	>50 ng/μl，OD260/280 介于 1.8-2.0 之間，無肉眼可見污染 ;	使用 Agilent Bioanalyzer 儀器檢測(cè)，則獲得的總的 RNA 28S：18S >1，RIN>7.0	請(qǐng)選擇DEPC 水保存樣品，并在樣品信息單中注明	樣品請(qǐng)置于 1.5 ml 管中，并使用封口膜封好，放置足夠的干冰運(yùn)輸	對(duì)于細(xì)菌和真菌樣品，希望盡量來自于同一個(gè)菌株，不要有宿主 DNA 污染

Turnaround time

The turnaround time for this service is 45 business days. The sequencing is performed on an Illumina Novaseq6000 platform using paired-end reads of 150 bp. The total raw data output is 12 Gb.

Results overview

Genome-wide coverage distribution plot: This plot shows the average depth of coverage for each chromosome (scaffold).

Sample PCA plot: This plot shows the distance between samples based on their read distribution. Samples that are far apart have significantly different read distributions, while samples that are close together have similar read distributions. PCA analysis is often used to assess the reproducibility of samples.

Differential gene volcano plot: This plot shows the expression levels of all genes in the sample, with significant differentially expressed genes highlighted.