Service introduction
Genome de novo sequencing, also known as de novo genome sequencing, is a method of sequencing the entire genome of a species without relying on a reference genome. This can be used to study the genome of a newly discovered species or to generate a more accurate and complete genome assembly of a species with a complex or highly repetitive genome.
Technical procedures
Services provided
We offer a variety of services related to genome de novo sequencing, including:
Genome survey: This service provides an overview of the genome, including its size, GC content, and repetitiveness.
Genome assembly statistics: This service provides information about the quality of the genome assembly, such as the average read depth and the N50 length of the contigs.
Genome annotation and gene function classification: This service assigns genes to functional categories and predicts their functions.
Comparative genomics and evolutionary analysis: This service compares the genome to other genomes to identify similarities and differences.
Assembly evaluation standards
Sample requirements
實驗類型 | DNA樣品總量 | 樣品濃度和純度 | 樣品保存 | 樣品質(zhì)量 |
動植物基因組de novo 測序 | 小片段文庫樣本制備需要總量大于 1 μg 的樣品,mate-pair 文庫(2 kb、5 kb、10 kb、)樣品,制備需要總量 20-50 μg | 樣品濃度 >200 ng/μl,OD260/280 介于 1.8-2.0 之間,無蛋白質(zhì)、RNA 或者肉眼可見雜質(zhì)污染 | 請保存于干粉、酒精、TE buffer 或超純水中,并請在樣品信息單中注明 | 基因組完整、無降解,電泳結(jié)果基因組 DNA 主帶應(yīng)在入 -Hind llldigest 最大條帶 23 KB 以上且主帶清晰,無彌散 |