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Main Page > Services > Next-generation sequencing > Animal and plant genomes de novo sequencing

Service introduction




        Genome de novo sequencing, also known as de novo genome sequencing, is a method of sequencing the entire genome of a species without relying on a reference genome. This can be used to study the genome of a newly discovered species or to generate a more accurate and complete genome assembly of a species with a complex or highly repetitive genome.

 

Technical procedures




 

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Services provided




        We offer a variety of services related to genome de novo sequencing, including:


        Genome survey: This service provides an overview of the genome, including its size, GC content, and repetitiveness.


        Genome assembly statistics: This service provides information about the quality of the genome assembly, such as the average read depth and the N50 length of the contigs.


        Genome annotation and gene function classification: This service assigns genes to functional categories and predicts their functions.


        Comparative genomics and evolutionary analysis: This service compares the genome to other genomes to identify similarities and differences.


 

Assembly evaluation standards




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Sample requirements




實驗類型

DNA樣品總量

樣品濃度和純度

樣品保存

樣品質(zhì)量

動植物基因組de novo 測序

小片段文庫樣本制備需要總量大于 1 μg 的樣品,mate-pair 文庫(2 kb、5 kb、10 kb、)樣品,制備需要總量 20-50 μg 

樣品濃度 >200 ng/μl,OD260/280 介于 1.8-2.0 之間,無蛋白質(zhì)、RNA 或者肉眼可見雜質(zhì)污染

請保存于干粉、酒精、TE buffer 或超純水中,并請在樣品信息單中注明

基因組完整、無降解,電泳結(jié)果基因組 DNA 主帶應(yīng)在入 -Hind llldigest 最大條帶 23 KB 以上且主帶清晰,無彌散

 


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020-89053723
Guangzhou IGE Biotechnology Co., Ltd.
Email: marketing@igebio.com
QQ: 1787730693
Address: Building G1-903, South China New Materials Innovation Park, #31 Kefeng Road, Huangpu Dist. Guangzhou, Guangdong prov. China 510663

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